By Janet K. Jansson
This ebook is an remarkable, very entire survey of equipment for tracking of particular microorganisms within the surroundings, with nice usefulness for these operating in both the review of hazards of published GMMs or with tracking of published microorganisms in e.g. soil bioremediation or biocontrol. the purpose is to supply a heritage in several thoughts for tracking of particular microorganisms in environmental samples in addition to examples of functions of those methods. The examples comprise paintings performed in laboratory version platforms (microcosms) in addition to documentation from real box releases of genetically changed microorganisms. The regulatory features of chance overview of genetically transformed microorganisms distinctive for liberate also are integrated. as a result, this publication is exclusive in facing diversified elements of tracking of GMMs and will be valuable for regulatory companies that require a precis of latest thoughts and data in addition to scientists that require a evaluate of the "state-of the artwork" of contemporary tools for tracking particular microorganisms in nature.
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Extra resources for Tracking Genetically-Engineered Microorganisms
Hirsch P, Spokes JD. Survival and dispersion of genetically modified rhizobia in the field and genetic interactions with native strains. FEMS Microbiol Ecol 1994; 15:147-160. 3. Kinkel LL, Wilson M, Lindow SE. Utility of microcosm studies for predicting phylloplane bacterium population sizes in the field. Appl Environ Microbiol 1996; 62:3413-3423. 4. Nielsen KM, Bones AM, van Elsas JD. Induced natural transformation of Acinetobacter calcoaceticus in soil microcosms. Appl Environ Microbiol 1997; 63:3972-3977.
Klingmuller, W. 1992. Risk assesment in releases of nitrogen-fixing Enterobacter into soil; survival and gene transfer, as influenced by agricultural substrates. In: Stewart-Tull DES, Sussman M, eds. The Release of Genetically Modified Microorganisms-REGEM 2. New York: Plenum Press,1992:155-157. 50. van Elsas JD. Antibiotic resistance gene transfer in the environment: an overview. In: Wellington EMH, van Elsas JD, eds. Genetic Interactions Among Microorganisms in the Natural Environment. Oxford: Pergamon Press, 1992; 17-39.
Recorbet et al15 determined the survival of an Escherichia coli population in soil using thenptII gene, and noted that the population decreased to < 102 after 15 days, as calculated by selective plate counts. However, by use of the most-probable-number-PCR method, 5 x 105 target sequences could still be detected after 40 days. It was not clear if this apparent population remaining in the soil was viable but uncultured or simply represented dead cells and extracellular DNA. 4. Choice of Antibiotic Resistance Gene Markers The choice of resistance gene markers depends on the environmental use of the marked bacterium and is influenced by the background resistance of the indigenous bacteria in a given habitat.
Tracking Genetically-Engineered Microorganisms by Janet K. Jansson