By Iain J. McEwan (auth.), Iain J. McEwan (eds.)
Acting mostly to regulate styles of gene expression, nuclear receptors play important roles in the course of embryonic improvement and within the rules of metabolic and reproductive services in grownup lifestyles, which proves this superfamily of ligand-activated transcription components to be an important a part of organic lifestyles. In The Nuclear Receptor Superfamily: tools and Protocols, specialist researchers describe various molecular, structural and telephone organic strategies presently used to enquire the structure-function of nuclear receptors, including experimental ways which may bring about new healing concepts for treating nuclear receptor-associated ailments. Written within the hugely winning Methods in Molecular Biology™ sequence structure, the chapters during this quantity include short introductions to the themes, lists of the mandatory fabrics and reagents, step by step, comfortably reproducible laboratory protocols, in addition to notes from the specialists to focus on pointers on troubleshooting and warding off identified pitfalls.
Cutting-edge and simple to take advantage of, The Nuclear Receptor Superfamily: tools and Protocols offers invaluable and time-saving tips for all these venture study during this ever-growing box of study.
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Additional resources for The Nuclear Receptor Superfamily: Methods and Protocols
Boldizsar F, Palinkas L, Czompoly T, Bartis D, Nemeth P, Berki T. Low glucocorticoid receptor (GR), high Dig2 and low Bcl-2 expression in double positive thymocytes of BALB/c mice indicates their endogenous glucocorticoid hormone exposure. Immunobiology 2006;211(10):785–96. W. Pike Abstract Crystallographic analysis of the ligand-binding domains of nuclear hormone receptors (NR) has provided a unique insight into the molecular events that underlie the ligand-mediated control of their transcriptional activity.
Isolation of purified subpopulations for further manipulation and investigation of functional capacity is also possible using a cell sorter, which uses similar technology to isolate cells for use by the researcher. This is especially important for studying responses of less abundant cell populations in tissues that express high levels of a target protein or receptor of interest. Furthermore, FACS analysis is clinically useful to identify and isolate responsive cell populations, which may be less appreciable in whole tissues because of the diluting effects of surrounding, nonresponding cell types.
FACSCalibur may require lines to be flushed if no events are being acquired by the flow cytometer. This may be due to the machine being clogged by clumps of cells or air bubbles that have formed. Other problems preventing the flow cytometer from acquiring events include a cracked sample tube (transfer cells to new tube); sheath reservoir too low or not properly capped (check fluid amount and placement of sheath cap); or waste reservoir being full (remove waste fluid). 50 Butts and Sternberg Acknowledgments This work was supported by the Intramural Research Program of the National Institute of Mental Health (NIMH)/NIH and a biodefense grant from the National Institute of Allergy & Infectious Diseases (NIAID)/NIH Intramural Research Program.
The Nuclear Receptor Superfamily: Methods and Protocols by Iain J. McEwan (auth.), Iain J. McEwan (eds.)