By Yutaka Kawarabayasi Ph.D. (auth.), Pratyoosh Shukla, Brett I. Pletschke (eds.)
Enzyme know-how is one the main promising disciplines in smooth biotechnology. during this ebook, the purposes of a large choice of enzymes are highlighted. present reports in enzyme expertise are targeted in the direction of the invention of novel enzymes (termed “bio-discovery” or “bio-prospecting”) and the id and elucidation of novel pathways of those novel enzymes with emphasis on their business relevance. With the advance of molecular strategies and different bioinformatics instruments, the time to combine this topic with different fields within the existence sciences has arrived. A speedy growth of the information base within the box of enzyme biotechnology has happened during the last few years. a lot of this growth has been pushed through the bio-discovery of many new enzymes from quite a lot of environments, a few severe in nature, by means of next protein (enzyme) engineering. those enzymes have came upon a variety of purposes, starting from bioremediation, bio-monitoring, biosensor improvement, bioconversion to biofuels and different biotechnologically very important value-added items. Hydrolases represent a huge part of the worldwide annual profit generated by way of business enzymes and the emphasis has hence been put on those enzymes and their purposes. With the gigantic curiosity of researchers energetic during this region, this ebook will serve to supply details on present facets during this box of analysis. within the present version, the contributions of many varied issues in the direction of developing new instructions of analysis within the quarter of enzyme biotechnology are defined. This booklet serves to supply a special resource of data to undergraduates, put up graduates and doctoral classes in microbiology and biotechnology besides allied lifestyles sciences. the current variation of the booklet covers all very important parts of enzyme biotechnology i.e. the wide range of enzymes within the box of enzyme biotechnology and their business functions, new equipment and state of the art info on smooth equipment of enzyme discovery. This e-book will act nearly as good source on lots of the present points of enzyme know-how for all scholars engaged in bioengineering and biotechnology.
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Process Biochem 37:267–274 Freitas FF, Marquez LDS, Ribeiro GP, Brandao GC, Cardoso VL, Ribeiro EJ (2011) A comparison of the kinetic properties of free and immobilized Aspergillus oryzae β-galactosidase. Biochem Eng J 58–59:33–38 Fujimoto H, Miyasato M, Ito Y, Sasaki T, Ajisaka K (1998) Purification and properties of recombinant β-galactosidase from Bacillus circulans. Glycoconj J 15:155–160 Gaur R, Pant H, Jain R, Khare SK (2006) Galactooligosaccharide synthesis by immobilized Aspergillus oryzae beta-galactosidase.
GOS Production with A. 11 illustrates the kinetics of GOS production using 400 g/l lactose and an enzyme concentration of 15 U/ml. 8 % of total carbohydrates in the mixture. From that point of maximum yield, the GOS concentration slowly decreased down to values of approximately 60 g/l. It has been proved that the increase of lactose concentration [up to 30–40 % (w/v)] has a strong positive effect on the maximum GOS obtained 35 3 On the Enzyme Specificity for the Synthesis of Prebiotic Galactooligosaccharides 400 Galactose Glucose Lactose Total GOS Concentration (g/L) 350 300 250 200 150 100 50 0 0 10 20 30 40 Reaction time (h) 50 60 Fig.
The permeabilizing agent may decrease the phospholipids content in the membrane thus allowing the transit of low molecular weight compounds in and out of the cells (Manera et al. 2010). B. Rodriguez-Colinas et al. In this context, ethanol-permeabilized K. lactis cells have been evaluated for lactose hydrolysis (Fontes et al. 2001; Genari et al. 2003; Panesar et al. 2007; Siso et al. 1992) and for the bioconversion of lactose and fructose to the disaccharide lactulose (Lee et al. 2004). We investigated the use of K.
Advances in Enzyme Biotechnology by Yutaka Kawarabayasi Ph.D. (auth.), Pratyoosh Shukla, Brett I. Pletschke (eds.)