Read e-book online Advances in Chromatography, Volume 35 PDF

By Phyllis R. Brown, Eli Grushka

ISBN-10: 058535796X

ISBN-13: 9780585357966

ISBN-10: 0824793617

ISBN-13: 9780824793616

"Volume 35 examines well timed matters resembling functionality requisites, detection modes, and ancillary concepts for optical detectors in capillary electrophoresis; and more."

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Extra resources for Advances in Chromatography, Volume 35

Sample text

In going to capillaries with even smaller internal diameters, however, the factor of improvement is more dramatic, as light coupling from commercial sources is extremely poor in such cases [31]. The final performance of the laser-based double-beam CE absorption system is quite impressive. The concentration LOD is 2 × 10-8 M of malachite green, or 2 × 10-16 mol injected [30]. This is the best transmission-based detection scheme reported so far. Actually, the electronic noise-cancellation circuit is capable of noise reduction to the 1 × 10-5 level when a larger flow cell is used.

Various Detection Modes 4 A. Absorption 4 B. Refractive Index 17 C. Fluorescence 20 D. Chemiluminescence 31 IV. Various Ancillary Techniques 32 A. Chemical Derivatization 32 B. Indirect Detection 35 C. Native Fluorescence of Biomolecules 38 D. On-Column Enzyme Assay 42 E. Particle-Counting Immunoassay 44 V. Summary 47 References 48 I Introduction After a relatively short period of development, capillary electrophoresis (CE) has become firmly established as a powerful separation tool [16]. Among the Page 2 many reasons for the rapid growth is the availability of user-friendly commercial instruments that derived much of the technology from familiar high-performance liquid chromatographic (HPLC) equipment.

When a laser beam is focused perpendicularly into the detection region of the capillary with a lens, interference of light results in the formation of dark and bright fringes surrounding the capillary axis in a plane perpendicular to it.  10 Instrumental optomechanical arrangement of the capillary refractive index detector. ) index of the medium in the detection region and the alignment. As a consequence, it is possible to measure the concentration of an analyte in the detection region by noting the change in the location of a given fringe.

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Advances in Chromatography, Volume 35 by Phyllis R. Brown, Eli Grushka

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